Enhancement

• Performs image enhancement, which helps bright objects to be separated from the background.
• Runs: Enhance contrast, median filter, sharpen algorithms.
• This improves the success rate of watershedding, and filling holes.

Threshold Pause

• A timesaving tool for thresholding large numbers of greyscale images.
• Operates on all images in a directory (there should not be any files in the directory that you don't want to threshold).
• The user sets the desired upper and lower greylevel values, then presses 'b', which performs the thresholding, and saves the binary image as a binary .gif (also adds a -bin extension to the filename).

Watershed_FillHoles

• Separates particles using the 'watershed' algorithm, then runs 'fill holes' to enclose white pixels that are completely enclosed by black pixels.

ROI Imager

• Draws ROI on an image. Requires a space delimited input file with x,y, width, height coordinates.
• This macro is useflu for checking that your ROI coordinate file is constructed properly.